Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantification of platelet activation status by analyzing P-selectin expression.

V Leytin1, M Mody, J W Semple

  • 1Department of Transfusion Medicine, St. Michael's Hospital and University of Toronto, Toronto, Ontario, Canada. valley@idirect.com

Biochemical and Biophysical Research Communications
|June 30, 2000
PubMed
Summary

Platelet activation status (PAS) measurement using P-selectin (CD62) expression varies significantly with flow cytometry parameters. Identifying sensitive parameters aids in diagnosing and monitoring platelet disorders.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revolutionizing Pediatric Neurophysiology With Magnetoencephalography.

Psychophysiology·2026
Same author

Durvalumab and tremelimumab plus local partial tumour ablation (radiofrequency ablation or stereotactic radiotherapy) in patients with unresectable liver metastases from metastatic colorectal cancer: results of the EORTC-1560-GITCG multicentre, single-arm phase II study (ILOC).

ESMO open·2025
Same author

Changes to country-specific hepatitis A travel vaccination recommendation for UK travellers in 2017-responding to a vaccine shortage in the national context.

Public health·2018
Same author

Antiplatelet antibody-induced thrombocytopenia does not correlate with megakaryocyte abnormalities in murine immune thrombocytopenia.

Scandinavian journal of immunology·2018
Same author

The impact of new national guidance for the public health management of enteric fever in England.

Public health·2017
Same author

High frequency jet ventilation for motion management during ablation procedures, a narrative review.

Acta anaesthesiologica Scandinavica·2017

Area of Science:

  • Hematology
  • Immunology
  • Biomedical Engineering

Background:

  • Platelet activation status (PAS) is crucial for assessing platelet quality and function in clinical and research settings.
  • Accurate characterization of PAS is essential for understanding platelet behavior in various conditions.

Purpose of the Study:

  • To investigate how different flow cytometric parameters affect the measurement of platelet activation status (PAS).
  • To identify sensitive parameters for distinguishing subtle differences in PAS among platelet populations.

Main Methods:

  • Platelet populations with varying PAS were generated using thrombin stimulation.
  • P-selectin (CD62) expression was measured using multiple flow cytometric parameters.
  • Analysis focused on identifying parameters sensitive to differences in CD62 expression.

Related Experiment Videos

Main Results:

  • PAS measurements showed significant variability (several-fold differences) depending on the specific flow cytometric parameters used.
  • Specific parameters were identified as more sensitive for differentiating between platelet populations with similar low or high PAS.
  • The choice of flow cytometry parameters critically influences the assessment of platelet activation.

Conclusions:

  • The selection of appropriate and sensitive flow cytometric parameters is vital for accurate PAS evaluation.
  • Improved PAS assessment can enhance the diagnosis and monitoring of platelet-associated disorders.
  • This study provides a foundation for optimizing flow cytometry protocols in platelet research and clinical diagnostics.