Related Experiment Video
Updated: Aug 18, 2026

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Published on: July 23, 2010
A functional NF-kappaB binding site in the human papillomavirus type 16 long control region
V Fontaine1, E van der Meijden, J de Graaf
1Department of Virology, AMC, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105 AZ, The Netherlands.
Abstract:
By computer search, we identified one potential NF-kappaB binding site in the HPV16 long control region (LCR) at position 7554-7563 having two mismatches in comparison to the consensus NF-kappaB binding site of the Igkappa L promoter. Bandshift experiments with nuclear extracts from HeLa cells or purified glutathione S-transferase-p65 fusion protein clearly demonstrated that NF-kappaB is able to bind to this region of the LCR. However, in comparison to NF-kappaB binding on a consensus probe, the affinity of NF-kappaB for this site is about 250-fold reduced. When mutations were introduced into this NF-kappaB binding site, the activity of the LCR was increased, strongly suggesting that NF-kappaB was acting as a transcriptional repressor in the context of the HPV16 LCR. In addition, overexpression of NF-kappaB p65 repressed the activity of the HPV16 LCR, strengthening this conclusion.
Related Concept Videos
Non-LTR Retrotransposons
Leaky Scanning
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Inhibitors Of Virion Release
Inhibitors of Virion Maturation and Assembly

