Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Increased polymorphonuclear leucocyte rigidity in HIV infected individuals.

A Tufail1, G N Holland, T C Fisher

  • 1UCLA Ocular Inflammatory Disease Center, the Jules Stein Eye Institute, and the Department of Ophthalmology, UCLA School of Medicine, Los Angeles, CA, USA.

The British Journal of Ophthalmology
|June 30, 2000
PubMed
Summary

Human immunodeficiency virus (HIV) infection increases polymorphonuclear leucocyte (PMN) rigidity, potentially contributing to HIV-related microvasculopathy. This increased rigidity is linked to immune dysfunction severity and CMV retinitis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omic spatial effects on high-resolution AI-derived retinal thickness.

Nature communications·2025
Same author

Deep masseteric layer.

British dental journal·2022
Same author

Biomarkers of systemic inflammation predict survival with first-line immune checkpoint inhibitors in non-small-cell lung cancer.

ESMO open·2022
Same author

Leaf cell membrane stability-based mechanisms of zinc nutrition in mitigating salinity stress in rice.

Plant biology (Stuttgart, Germany)·2017
Same author

Clinical audit in retina 2016: Chairman's introduction.

Eye (London, England)·2017
Same author

The UK Neovascular AMD Database Report 3: inter-centre variation in visual acuity outcomes and establishing real-world measures of care.

Eye (London, England)·2016

Area of Science:

  • Immunology
  • Hematology
  • Virology

Background:

  • Human immunodeficiency virus (HIV) infection is associated with various complications affecting blood flow.
  • Polymorphonuclear leucocytes (PMNs) play a crucial role in immune response and microvascular integrity.
  • Altered PMN function may contribute to the pathogenesis of HIV-related conditions.

Purpose of the Study:

  • To investigate polymorphonuclear leucocyte (PMN) rigidity in individuals with HIV infection.
  • To determine if PMN rigidity is associated with cytomegalovirus (CMV) retinitis in HIV-infected individuals.
  • To explore the relationship between PMN rigidity, immune status (CD4+ T lymphocyte count), and HIV-related microvasculopathy.

Main Methods:

  • Measured PMN rigidity using cell transit time through 8-micrometer pores in a cell transit analyser.

Related Experiment Videos

  • Compared PMN transit times between HIV-infected individuals (with and without CMV retinitis) and HIV-negative controls.
  • Analyzed correlations between PMN transit times, CD4+ T lymphocyte counts, and clinical parameters.

    • Main Results:

    • PMNs from HIV-infected individuals exhibited significantly longer transit times, indicating increased rigidity, compared to controls (p<0.001).
    • PMNs from HIV-infected individuals with CMV retinitis showed greater rigidity than those without CMV retinitis (p<0.001).
    • Increased PMN rigidity was significantly correlated with lower CD4+ T lymphocyte counts (p<0.001).

    Conclusions:

    • HIV infection is associated with increased PMN rigidity, a potential factor in HIV-related retinal microvasculopathy.
    • PMN rigidity severity correlates with the degree of immune dysfunction in HIV-infected individuals.
    • Elevated PMN rigidity may persist in patients with CMV retinitis even after effective antiretroviral therapy improves CD4+ counts.