Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virulence gene regulation inside and outside.

V J DiRita1, N C Engleberg, A Heath

  • 1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48103-0620, USA. vdirita@umich.edu

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|June 30, 2000
PubMed
Summary

Microbial virulence gene regulation differs between Vibrio cholerae and Streptococcus pyogenes. V. cholerae uses complex induction, while S. pyogenes virulence is enhanced by a lack of regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and methods of the REMOVAL-HD study: a tRial Evaluating Mid cut-Off Value membrane clearance of Albumin and Light chains in HaemoDialysis patients.

BMC nephrology·2018
Same author

Alkaliphilic Bacillus species show potential application in concrete crack repair by virtue of rapid spore production and germination then extracellular calcite formation.

Journal of applied microbiology·2017
Same author

Investigation of circadian disruption in bipolar disorder.

Acta neuropsychiatrica·2016
Same author

Collaborative study for the establishment of the WHO 3(rd) International Standard for Endotoxin, the Ph. Eur. endotoxin biological reference preparation batch 5 and the USP Reference Standard for Endotoxin Lot H0K354.

Pharmeuropa bio & scientific notes·2016
Same author

Randomised clinical trial: vercirnon, an oral CCR9 antagonist, vs. placebo as induction therapy in active Crohn's disease.

Alimentary pharmacology & therapeutics·2015
Same author

Factor VIII Bypassing Activity (FEIBA) assays: standardization and development of the 1st NIBSC Working Standard for FEIBA--results from a collaborative study.

Haemophilia : the official journal of the World Federation of Hemophilia·2012

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Gene Regulation

Background:

  • Understanding microbial virulence is crucial for combating infectious diseases.
  • Most knowledge on microbial gene regulation and virulence comes from in vitro studies.
  • This review correlates in vitro and in vivo observations for two distinct bacterial pathogens.

Purpose of the Study:

  • To compare and contrast the regulatory mechanisms of virulence in Vibrio cholerae and Streptococcus pyogenes.
  • To correlate in vitro findings with in vivo observations of bacterial gene expression and pathogenicity.

Main Methods:

  • Comparative analysis of gene regulation in Vibrio cholerae and Streptococcus pyogenes.
  • Review of in vitro and in vivo experimental data on bacterial virulence factors.

Related Experiment Videos

  • Examination of regulatory pathways, including transcription activators and two-component systems.

    • Main Results:

    • Vibrio cholerae exhibits a complex regulatory cascade for virulence gene expression (toxin and pilus) in vivo, yet is downregulated by host signals like bile and temperature.
    • Streptococcus pyogenes virulence, specifically capsule production, is enhanced by the absence of regulation through the CsrR and CsrS two-component system.
    • Inner membrane proteins in V. cholerae may play a role in signal transduction for virulence, but current models might be oversimplified.

    Conclusions:

    • Vibrio cholerae has evolved intricate induction-based regulation for in vivo virulence.
    • Streptococcus pyogenes demonstrates a contrasting strategy where reduced regulation potentiates pathogenicity.
    • The study highlights diverse evolutionary paths in bacterial adaptation and virulence.