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Interaction between alpha-MSH and acetylcholinergic system upon striatal cAMP and IP(3) levels
M C Cremer1, S R de Barioglio, M E Celis
1Laboratorio de Fisiología, Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, CP 5000, Córdoba, Argentina.
Peptides
|July 6, 2000
Summary
Alpha-melanocyte-stimulating hormone (alpha-MSH) interacts with the cholinergic system in the striatum, influencing cyclic adenosine monophosphate (cAMP) and inositol triphosphate (IP3) production. This interaction involves M1 and nicotinic receptors, suggesting related intracellular signaling pathways.
Area of Science:
- Neuroscience
- Molecular Biology
- Pharmacology
Background:
- The neuropeptide alpha-MSH and the cholinergic system play roles in striatal function.
- Cholinergic drugs are known to induce dopamine release in the striatum.
- Dopamine D1 receptors are implicated in modulating intracellular signaling pathways.
Purpose of the Study:
- To investigate the interaction between alpha-MSH and the cholinergic system in rat striatal slices.
- To examine the role of D1 receptors in alpha-MSH and cholinergic-stimulated cAMP and IP3 production.
- To elucidate the involvement of muscarinic and nicotinic receptors in these interactions.
Main Methods:
- In vitro study using rat striatal slices.
- Measurement of cyclic adenosine monophosphate (cAMP) and inositol 1-3-5 triphosphate (IP3) production.
- Administration of alpha-MSH, cholinergic agents (pilocarpine, McN-A-343), and receptor antagonists (SCH-23390, gallamine, pirenzepine, di-hydro-beta-eritroidine).
Main Results:
- Alpha-MSH, pilocarpine, and McN-A-343 increased striatal cAMP and IP3 levels, effects largely blocked by the D1 antagonist SCH-23390.
- Gallamine increased cAMP, while pirenzepine decreased both cAMP and IP3, and di-hydro-beta-eritroidine decreased cAMP.
- Simultaneous administration of alpha-MSH and cholinergic agents modified cAMP and IP3 levels, indicating an interaction via M1 and nicotinic receptors.
Conclusions:
- Alpha-MSH interacts with the striatal cholinergic system through M1 and nicotinic receptors.
- These interactions influence intracellular signaling pathways involving cAMP and IP3 production.
- The findings suggest a relationship between the signaling pathways modulated by alpha-MSH and cholinergic receptors in the striatum.