The interactions between juvenile hormone (JH), lipophorin, vitellogenin, and JH esterases in two cockroach species

For the cockroach species Leucophaea maderae and Periplaneta americana two major juvenile hormone (JH)-binding proteins have been identified: lipophorin (Lp) and vitellogenin (Vg). Each of these macromolecules binds JH with an approximate affinity of K(d) of 10 nM. In Leucophaea the concentration of Lp is augmented by JH during vitellogenesis at the same time when Vg is induced de novo. The circulating levels of each of Lp and Vg at mid-vitellogenesis are in the 10 microM range. Similar values have been determined for Periplaneta. Total JH concentrations (bound and free) can be as high as micromolar in Leucophaea. However, because of the large quantities of the two major JH-binding proteins and their high affinity for JH, we can assume that the amount of free (unbound) JH in circulation is extremely low (the actual values are not know). The JH esterases (JHEs) of the hemolymph in both cockroach species have been isolated by anion exchange chromatography. The JHEs of Leucophaea bound to the anion exchange resin more tightly than the JHE of Periplaneta. The V(max) of the Leucophaea esterases fluctuated by a factor of 2 to 3 during vitellogenesis. The K(m) values for the two distinct esterases of Leucophaea were similar (about 0.15x10(-6) M). On the other hand, k(cat) of the JHEs for Leucophaea at ovulation time was two to three times higher than earlier during vitellogenesis, i.e. 23.30 min(-l) compared to 6.20 min(-1). The JHE of Leucophaea is shown to bind JH III with high affinity: K(d)=3x10(-9) M. However, since there are only very small amounts of JH available for degradation (due to the binding to Lp and Vg), the quantitative removal of JH from circulation, and this includes the release of bound JH, is indeed slow, with a measured half-life of 6-8 h. Classical kinetic assumptions are not met in conditions where the enzyme concentrations exceed by far that of the available substrate. Nonetheless, we attempted to determine the initial velocity of JH hydrolysis under natural conditions, i.e. for undiluted hemolymph, by measuring the initial velocities of JH hydrolysis in serially diluted hemolymph and extrapolating to zero dilution. For in vivo conditions we estimated an initial velocity of JH hydrolysis of <0.1 fmol microl hemolymph(-1) min(-1), i.e. four to five orders of magnitude lower than that measured at substrate saturation in vitro.