[Characterization and stage-specific change of proteins during the embryonic development of silkworm Bombyx mori]

The gene expression pattern was analysed employing protein two-dimensional electrophoresis and amino acid sequencing during the embryonic development of silkworm Bombyx mory. The 2D-PAGE pattern of the silkworm embryonic proteins had little change in the long developmental period from the Critical Stage until the Head Pigmentation Stage of silkworm embryo. The percentage of matched proteins that found between the spots in the 2D-PAGE pattern of the Critical Stage of silkworm embryo and the spots in the 2D-PAGE pattern of the Head Pigmentation Stage of silkworm embryo was 63%. Silkworm Egg-specific Protein and 30K Protein were in large amount. However, 2D-PAGE pattern of the silkworm embryonic proteins showed considerably varied in shorter developmental stage from the Head Pigmentation Stage to the Body Pigmentation Stage and until the Newly Hatched Larva. The percentage of matched proteins that found the spots in the 2D-PAGE pattern of each developmental stage of silkworm embryo became lesser. The protein spots that the isoelectric points lying on the acidic side greatly increased, the Silkworm Egg-specific Protein and 30K Protein were gradually disappeared and the gene that resembles BmLSP's, Enolase's and Superoxide dismutase's gene was activated and expressed with the embryonic development.