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Updated: Jul 14, 2026

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
Interaction of phospholipid bilayers with polyamines of different length
A Zheliaskova1, S Naydenova, A G Petrov
1Biomolecular Layers Department, Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia. georgant@issp.bas.bg
Abstract:
The tip-dip patch clamp method was used to study the effect of three polyamines, putrescine, spermidine and spermine, on bilayer lipid membrane (BLM) stability. Two kinds of mixed lipid-polyamine membranes were investigated. The poration voltage (V(p)), and the closed (sigma(cl)) and open (sigma(op)) state conductances for pure and polyamine-treated lipid membranes were determined by the method of current-voltage surfaces. It was demonstrated that putrescine and spermidine destabilized lipid membranes under all circumstances. BLM stabilization by spermine was observed when it was added to preformed membranes.
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