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beta-Galactosidase immobilization for milk lactose hydrolysis: a simple experimental and modelling study of batch and
1Department of Biochemistry and Molecular Biology B, Faculty of Chemistry, University of Murcia, P.O. Box 4021, E-30100, Murcia, Spain
Abstract:
The experiment described in this paper introduces students to the practical use of an enzyme (beta-galactosidase, or lactase) acting on a natural substrate. The enzyme is immobilized onto a cheap support, and the immobilized derivative is used in a packed-bed reactor for continuous milk lactose hydrolysis. The results are compared to those obtained for discontinuous batch reactors with soluble enzyme. A mathematical model of the two types of reactors is run, and its results are compared with the experimental data obtained.
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