Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phosphodiester Linkages

Phosphodiester Linkages

Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...

Ligand Binding and Linkage

Ligand Binding and Linkage

No description available

Cancer-Critical Genes II: Tumor Suppressor Genes

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Cancer Survival Analysis

Cancer Survival Analysis

Cancer survival analysis focuses on quantifying and interpreting the time from a key starting point, such as diagnosis or the initiation of treatment, to a specific endpoint, such as remission or death. This analysis provides critical insights into treatment effectiveness and factors that influence patient outcomes, helping to shape clinical decisions and guide prognostic evaluations. A cornerstone of oncology research, survival analysis tackles the challenges of skewed, non-normally...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impaired tetramer assembly of variant medium-chain acyl-coenzyme A dehydrogenase with a glutamate or aspartate substitution for lysine 304 causing instability of the protein.

The Journal of biological chemistry·1992

Same author

The prognostic value of cytogenetic analyses in patients with acute nonlymphocytic leukemia treated with the same intensive chemotherapy.

Cancer·1992

Same author

Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination.

Nature·1992

Same author

[Antitumor effect of locally injected TNF.fibrin gel against murine colon 26 subcutaneous tumor: preliminary report].

Nihon Geka Gakkai zasshi·1992

Same author

[Clinical studies on cefprozil granules in pediatric skin soft tissues infections].

The Japanese journal of antibiotics·1992

Same author

Generalized resistance to thyroid hormone (GRTH) in a family: case studies.

Endocrinologia japonica·1992

Search research articles

Related Experiment Video

Updated: Feb 2, 2026

Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

Published on: June 9, 2018

[Linkage analysis for identifying genes implicated in hereditary cancers].

M Sekine¹, K Tanaka

¹Department of Obstetrics and Gynecology, Niigata University School of Medicine.

Nihon Rinsho. Japanese Journal of Clinical Medicine

|July 6, 2000

Summary

Linkage analysis helps identify genes for hereditary cancers like breast and colorectal cancer. Nonparametric methods are effective for genetic mapping by focusing on shared alleles among affected individuals.

More Related Videos

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Published on: August 16, 2017

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

Related Experiment Videos

Last Updated: Feb 2, 2026

Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

Genetic Analysis of Hereditary Transthyretin Ala97Ser Related Amyloidosis

Published on: June 9, 2018

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Published on: August 16, 2017

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

Area of Science:

Human Molecular Genetics
Cancer Genetics
Genetic Epidemiology

Context:

Identifying genes for hereditary cancers (e.g., retinoblastoma, colorectal, breast) has been achieved using positional cloning.
Linkage analysis is a key method for disease gene localization.
Parametric linkage analysis requires a precise genetic model, unlike nonparametric methods.

Purpose:

To detail the rationale and strategy of linkage analysis for hereditary cancer gene mapping.
To explain the application of nonparametric linkage analysis in identifying disease genes.
To provide a comprehensive overview of genetic mapping techniques for inherited cancer syndromes.

Summary:

Linkage analysis, particularly nonparametric methods, is crucial for locating disease genes in hereditary cancers.
Nonparametric methods identify disease genes by analyzing shared alleles among affected individuals, bypassing the need for a predefined genetic model.
This approach has been successfully applied to numerous hereditary diseases and is detailed herein for cancer genetics.

Impact:

Facilitates the identification of novel cancer susceptibility genes.
Improves understanding of the genetic basis of hereditary cancers.
Aids in the development of targeted genetic testing and personalized cancer prevention strategies.