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Related Experiment Video

Updated: Jun 26, 2026

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
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Published on: September 18, 2014

Cellular requirements for simulation by the purified streptococcal antigen DSR.

R H Tomar, R Knight

    International Archives of Allergy and Applied Immunology
    |January 1, 1976
    PubMed
    Summary
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    T lymphocytes play a crucial role in the delayed skin reaction (DSR) to streptokinase-streptodornase. Removing T cells significantly reduced the immune response, while B cell depletion had no effect.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • The delayed skin reaction (DSR) is a key indicator of cellular immune response.
    • Streptokinase-streptodornase (SKSD) is an antigen used to elicit DSR.
    • Understanding the specific immune cell populations involved in DSR is crucial for immunological studies.

    Purpose of the Study:

    • To investigate the role of different peripheral blood mononuclear cell subsets in the delayed skin reaction (DSR) induced by streptokinase-streptodornase (SKSD).
    • To determine whether T cells, B cells, or monocytes are essential for the DSR response.

    Main Methods:

    • Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Hypaque density gradient centrifugation.
    • Cell populations were depleted of T cells (via sheep erythrocyte rosette formation), B cells (via nylon-wool column adsorption), and monocytes (via adherence and iron uptake).

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  • The proliferative response to DSR antigen was measured by tritiated thymidine incorporation into DNA.

    • Main Results:

    • Depletion of T lymphocytes significantly diminished the DSR response.
    • Removal of B lymphocytes did not affect the DSR response.
    • Monocyte-depleted cells showed reduced tritiated thymidine incorporation in both antigen-stimulated and control conditions, but the stimulation index remained unchanged.

    Conclusions:

    • T lymphocytes are critical for mediating the delayed skin reaction to streptokinase-streptodornase.
    • B lymphocytes do not play a significant role in this specific DSR.
    • Monocytes contribute to the overall cellular proliferation but do not alter the antigen-specific stimulation index in this context.