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Related Experiment Videos

E-selectin expression and stimulation by inflammatory mediators are developmentally regulated during embryogenesis.

D S Milstone1, P E O'Donnell, G Stavrakis

  • 1Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA. milstone@rascal.med.harvard.edu

Laboratory Investigation; a Journal of Technical Methods and Pathology
|July 6, 2000
PubMed
Summary

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The study reveals distinct developmental stages for endothelial adhesion molecule expression during embryogenesis. E-selectin inducibility emerges later than intercellular adhesion molecule-1 (ICAM-1), indicating differential regulation in developing blood vessels.

Area of Science:

  • Developmental Biology
  • Immunology
  • Vascular Biology

Background:

  • Leukocyte recruitment during inflammation depends on endothelial adhesion molecules.
  • E-selectin and ICAM-1 are key adhesion molecules involved in inflammatory responses.
  • Understanding their developmental regulation is crucial for comprehending immune cell trafficking in embryos.

Purpose of the Study:

  • To investigate the developmental onset of E-selectin and ICAM-1 expression and inducibility.
  • To analyze the ontogeny of endothelial adhesion molecule expression in murine embryos and embryoid bodies (EB).
  • To identify distinct stages in the development of E-selectin inducibility.

Main Methods:

  • Studied murine embryos at various developmental stages (E9.5-E13.5).

Related Experiment Videos

  • Utilized embryoid bodies (EB) derived from embryonic stem cells to model early endothelial development.
  • Assessed basal and induced expression of E-selectin and ICAM-1 using inflammatory mediators and endotoxin.

    • Main Results:

    • E-selectin and ICAM-1 exhibit differential regulation during development.
    • Three distinct stages characterize the ontogeny of E-selectin inducibility.
    • ICAM-1 inducibility is present earlier than E-selectin inducibility in both embryos and EB.
    • Embryos are protected from maternal inflammatory insults.
    • E-selectin expression is inducible starting from embryonic day 12.5-13.5.

    Conclusions:

    • The acquisition of E-selectin inducibility is a developmentally regulated process.
    • This regulation likely involves changes in signal transduction, transcription factors, and chromatin accessibility.
    • Specific endothelial subpopulations may exhibit restricted inducibility of E-selectin.