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Updated: Aug 18, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Electrically driven microseparation methods for pesticides and metabolites: III. Capillary electrochromatography with
1Department of Chemistry, Oklahoma State University, Stillwater 74078-3071, USA.
Abstract:
A silica-based stationary phase with surface bound silylpropyl trialkylammonium functions was introduced and evaluated in the capillary electrochromatography of alkylbenzenes and pesticides. This stationary phase is referred to as octadecyldimethyl(3-trimethoxysilylpropyl) ammonium-silica (ODAS) and has quaternary amine functions that generate an anodic electroosmotic flow (EOF) and octadecyl functions that are responsible for solute retention by a reversed-phase chromatography mechanism. The ODAS stationary phase was characterized over a wide range of elution conditions in term of EOF and retention behavior of alkylbenzene homologous series. The ODAS stationary phase proved useful in the separation of pesticides as well as in the on-column preconcentration of dilute pesticide samples, thus permitting the detection of solution at 7 x 10(-7) M using a UV detector.
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