Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Solid-phase synthesis of cyclic peptide-DNA hybrids.

C F Bleczinski¹, C Richert

¹Department of Chemistry, Tufts University, Medford, MA 02155, USA.

Organic Letters

|July 6, 2000

Summary

Researchers developed a new method for creating cyclic peptide-DNA hybrids. These hybrids show high resistance to enzymes, making them promising for various applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthesis of an oligonucleotide with a nicotinamide mononucleotide residue and its molecular recognition in DNA helices.

Organic & biomolecular chemistry·2015

Same author

Effect of preorganization on the affinity of synthetic DNA binding motifs for nucleotide ligands.

Organic & biomolecular chemistry·2015

Same author

A steroid cap adjusts the selectivity and accelerates the rates of nonenzymatic single nucleotide extensions of an oligonucleotide.

Journal of the American Chemical Society·2001

Same author

Redesigning nucleic acids.

Pure and applied chemistry. Chimie pure et appliquee·2001

Same author

Synthesis and monitored selection of nucleotide surrogates for binding T:A base pairs in homopurine-homopyrimidine DNA triple helices.

Nucleic acids research·2001

Same author

Monitored selection of DNA-hybrids forming duplexes with capped terminal C:G base pairs.

Journal of combinatorial chemistry·2001

Area of Science:

Bioconjugation Chemistry
Oligonucleotide Synthesis
Peptide Chemistry

Background:

Peptide-DNA hybrids offer unique properties for molecular recognition and therapeutics.
Developing efficient synthesis methods for these complex molecules remains a challenge.

Purpose of the Study:

To report a novel methodology for the high-yield preparation of cyclic peptide-DNA hybrids.
To demonstrate the suitability of this method for combinatorial library synthesis.

Main Methods:

Utilized Fmoc/Alloc-protected amino acid and nucleoside phosphoramidites.
Employed an omega-hydroxylauric acid-derivatized support on controlled pore glass.
Synthesized cyclic hybrids, including a Glu-Leu-TT-DP-Lys sequence with 5'-amino-5'-deoxynucleotide modifications.

Main Results:

Achieved high yields in the preparation of cyclic peptide-DNA hybrids.
Demonstrated the applicability of the method for library synthesis.
The synthesized cyclic hybrid (Glu-Leu-TT-DP-Lys) displayed significant resistance to both exo- and endonucleases.

Conclusions:

The reported methodology provides an efficient route to cyclic peptide-DNA hybrids.
These hybrids possess enhanced nuclease resistance, suggesting potential in nuclease-rich environments.
The method is amenable to the generation of diverse peptide-DNA hybrid libraries.

Related Experiment Videos