Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cross-talk between olfactory second messenger pathways.

A Vogl1, J Noé, H Breer

  • 1University Stuttgart-Hohenheim, Institute of Physiology, Stuttgart, Germany.

European Journal of Biochemistry
|July 6, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Updated overall survival and final progression-free survival data for patients with treatment-naive advanced ALK-positive non-small-cell lung cancer in the ALEX study.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

Expression of odorant-binding proteins in mouthpart palps of the desert locust Schistocerca gregaria.

Insect molecular biology·2018
Same author

Larval sensilla of the moth Heliothis virescens respond to sex pheromone components.

Insect molecular biology·2016
Same author

The sensory neurone membrane protein SNMP1 contributes to the sensitivity of a pheromone detection system.

Insect molecular biology·2014
Same author

Thirty years of mercurial diuretics; a review of their history and present status.

Medical record (New York : 1934)·2014
Same author

Some newer disease entities.

Proceedings of the Rudolf Virchow Medical Society in the City of New York·2014
Same journal

Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

European journal of biochemistry·2020
Same journal

AB 3.1.1.1 (or EC 3.1.1.?).

European journal of biochemistry·2020
Same journal

Cdk5.

European journal of biochemistry·2018
Same journal

Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

European journal of biochemistry·2004
Same journal

Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

European journal of biochemistry·2004
Same journal

Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

European journal of biochemistry·2004
See all related articles

Odor signals in rats involve two pathways, cyclic AMP (cAMP) and inositol trisphosphate (InsP3), which functionally oppose each other. Protein kinase A mediates cAMP

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Olfactory signal transduction relies on second messengers like cyclic AMP (cAMP) and inositol trisphosphate (InsP3).
  • These pathways are crucial for detecting odors in various species.
  • Previous research suggested independent functioning of these signaling cascades.

Purpose of the Study:

  • To investigate the interaction between cAMP and InsP3 signaling pathways in rat olfactory neurons.
  • To determine if these pathways operate independently or exhibit functional antagonism.
  • To elucidate the molecular mechanisms underlying the cross-talk between cAMP and InsP3 signaling.

Main Methods:

  • Isolated olfactory cilia from rats were used.
  • Pharmacological agents U-73122 (phospholipase C inhibitor) and MDL-12,330 A (adenylate cyclase inhibitor) were employed.

Related Experiment Videos

  • Odor-induced signaling was measured, and the role of protein kinase A (PKA) was assessed.

    • Main Results:

    • Inhibition of phospholipase C (PLC) augmented odor-induced cAMP signaling.
    • Activation of the phosphoinositol pathway attenuated odor-induced cAMP formation.
    • Elevated cAMP suppressed odor-induced InsP3 signaling, mediated by PKA, not InsP3 degradation.

    Conclusions:

    • The cAMP and InsP3 signaling pathways in rat olfactory neurons are not independent but functionally antagonistic.
    • Cross-talk between these pathways involves inhibition of PLC activation by elevated cAMP, likely via PKA.
    • This antagonism provides a more complex regulatory mechanism for olfactory signal transduction.