Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Morphine-associated environmental cues elicit conditioned gene expression.

B E Schroeder1, M R Holahan, C F Landry

  • 1Neuroscience Training Program, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA. beschroe@students.wisc.edu

Synapse (New York, N.Y.)
|July 6, 2000
PubMed
Summary

Drug cues trigger conditioned behaviors by activating brain circuits like the prefrontal cortex. This Fos expression, indicating neural activity, fades within two weeks, offering insights into drug addiction relapse.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cerebral amyloid angiopathy: an update.

Journal of neurology·2023
Same author

Acute postnatal exposure to di(2-ethylhexyl) phthalate adversely impacts hippocampal development in the male rat.

Neuroscience·2011
Same author

Measurement of rodent stereotyped behavior.

Current protocols in neuroscience·2008
Same author

Profiles of novel diurnally regulated genes in mouse hypothalamus: expression analysis of the cysteine and histidine-rich domain-containing, zinc-binding protein 1, the fatty acid-binding protein 7 and the GTPase, ras-like family member 11b.

Neuroscience·2006
Same author

Differential patterns of extracellular signal-regulated kinase-1 and -2 phosphorylation in rat limbic brain regions after short-term and long-term inhibitory avoidance learning.

Neuroscience·2005
Same author

Instrumental learning, but not performance, requires dopamine D1-receptor activation in the amygdala.

Neuroscience·2005

Area of Science:

  • Neuroscience
  • Behavioral Pharmacology
  • Molecular Biology

Background:

  • Drug-associated environmental cues powerfully influence behavior through learned associations.
  • The specific brain circuits and molecular mechanisms underlying these drug-environment associations remain incompletely understood.

Purpose of the Study:

  • To investigate the neural substrates of drug-associated conditioned behavior.
  • To examine the expression of the immediate early gene Fos in specific brain regions following drug conditioning.

Main Methods:

  • A drug-conditioning paradigm was employed using morphine or saline administered daily for 10 days, paired with a distinct environment.
  • Immunocytochemistry was used to detect Fos expression, a marker of neural activity, in rat brain tissue.
  • Behavioral activation and motor responses were monitored after mock injections in the drug-paired environment at various time points post-treatment.

Related Experiment Videos

Main Results:

  • Conditioned behavioral activation was observed 3, 5, and 7 days after morphine treatment.
  • Significant increases in Fos-positive cells were found in the prefrontal cortex, cingulate cortex, nucleus accumbens, and preoptic area in rats exhibiting the conditioned motor response.
  • Fos expression was not directly correlated with motor activity and diminished by 7 days, becoming absent by 14 days post-treatment.

Conclusions:

  • Specific cortical and limbic brain areas are activated by drug-associated cues, mediating conditioned behavioral responses.
  • The observed Fos expression patterns suggest a time-limited neural basis for drug-environment associations.
  • Understanding these neural mechanisms is crucial for addressing relapse in drug addiction.