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Updated: Oct 15, 2026

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport
Published on: March 6, 2015
Bacterial translocation in obstructive jaundice in rats: role of mucosal lacteals
D J Kordzaya1, V T Goderdzishvili
1First Department of Surgery, Institute of Surgery, Tbilisi, Georgia.
Objective:
To study the ultrastructure of the ileal wall in rats with obstructive jaundice alone and after passive external biliary drainage to see if we could discover the reason for the increased risk of infective complications and multisystem failure in the presence of obstructive jaundice and after external biliary drainage.
Design:
Histological examination of the wall of the terminal ileum using light microscopy, as well as scanning and transmission electron microscopy (EM).
Setting:
Experimental laboratory, Republic of Georgia.
Animals:
56 adult male Wistar rats.
Interventions:
Rats were divided into 7 groups: controls (not operated on, n = 6); sham-operated and studied after 6 and 12 days (n = 6 in each); bile duct ligation alone studied after 6 and 12 days (n = 10 in each); and bile duct ligation followed 6 and 12 days later by one-day of external biliary drainage (n = 9 in each).
Main Outcome Measures:
Percentage of destroyed villi.
Results:
The extent of oedema of villi, necrosis of neurons, and disturbances in the secretory capacity of enterocytes correlated well with the duration of cholestasis. After 6 and 12 days ligation alone 7.2% and 17.3%, respectively, of villi were found to be destroyed; their connective tissue framework including lymphatics was in direct contact with the intestinal contents. The changes were not reversed by one day of external biliary drainage.
Conclusion:
The gaps in the ileal mucosa caused by obstructive jaundice (and not relieved by one day of external biliary drainage) may enable gut bacteria and their endotoxin to reach the systemic circulation through the lymphatic-system. This could increase the risk of infective complications.
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