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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Phosphatidylserine/Cholesterol Bilayers Supported on a Polycation/Alkylthiol Layer Pair
1Center on Polymer Interfaces and Macromolecular Assemblies (CPIMA), Department of Chemical Engineering and Materials Science, University of California at Davis, Davis, California, 95616
Abstract:
1-Stearoyl-2-oleoyl phosphatidylserine (SOPS)/cholesterol bilayers, supported on a polycation/alkylthiol layer pair on a gold surface, were investigated by surface plasmon resonance (SPR) and fluorescence recovery after photobleaching. The substrate was formed by electrostatic adsorbance of a hydrated poly(diallyldimethylammonium chloride) (PDDA) layer on the negatively charged surface of a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) on gold. Lipid membranes with different SOPS/cholesterol compositions were deposited on the PDDA/MUA layer pair by vesicle fusion. When the cholesterol content was below 20%, single bilayers were deposited. Fluorescence recovery after the bleaching experiments revealed that the SOPS/cholesterol bilayers were mobile at room temperature; lateral diffusion coefficients of a fluorescence probe were approximately 1x10(-9) cm(2)/s. The kinetics of the addition of the ion-channel-forming peptide gramicidin to the supported bilayers was detected by SPR. Copyright 2000 Academic Press.
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