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Viability and functional activity of cryopreserved mononuclear cells
1University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. adriana.weinberg@uchsc.edu
Clinical and Diagnostic Laboratory Immunology
|July 7, 2000
Summary
Cryopreserved peripheral blood mononuclear cells (PBMC) viability impacts function. Higher viability, often seen with experienced staff, ensures reliable lymphocyte proliferation responses for functional analyses.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Cryopreservation of peripheral blood mononuclear cells (PBMC) is crucial for immunological studies.
- Maintaining PBMC viability and function post-thaw is essential for reliable experimental outcomes.
- Factors affecting PBMC quality in multi-center trials require investigation.
Purpose of the Study:
- To identify factors influencing the viability and function of cryopreserved PBMC.
- To assess the relationship between PBMC viability and lymphocyte proliferation.
- To establish viability thresholds for successful functional assays.
Main Methods:
- Analysis of 54 PBMC samples from 27 AIDS Clinical Trial Units.
- Assessment of PBMC viability using standard laboratory techniques.
- Measurement of lymphocyte proliferation to evaluate PBMC function.
Main Results:
- PBMC viability varied widely (1-96%), with a median of 70%.
- Higher viability was correlated with experienced laboratory staff.
- Lymphocyte proliferation, a measure of function, was directly associated with PBMC viability.
Conclusions:
- PBMC viability is a critical determinant of their functional capacity.
- A viability threshold of >= 70% is recommended for consistent and reliable functional analyses.
- Laboratory experience significantly influences PBMC cryopreservation quality.