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Related Experiment Videos

Cell death in vasculitic neuropathy.

D Heuss1, S Probst-Cousin, C Kayser

  • 1Department of Neurology, Friedrich Alexander University of Erlangen-Nürnberg, Schwabachanlage 6, D-91054 Erlangen, Germany. dieter.heuss@neuro.med.uni-erlangen.de

Muscle & Nerve
|July 7, 2000
PubMed
Summary

Vasculitic neuropathy involves CD8+ T-cells inducing apoptosis via granzymes/TIA-1 or Fas/Fas-ligand. Apoptosis in these inflammatory cells suggests a role in recovery from vasculitic neuropathy.

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Area of Science:

  • Immunology
  • Neurology
  • Cell Biology

Background:

  • Vasculitic neuropathy is characterized by perivascular infiltrates of CD4+ and CD8+ T-cells.
  • CD8+ T-cells employ cytotoxic mechanisms, including granzyme/TIA-1 release and Fas/Fas-ligand interactions, to induce apoptosis.

Purpose of the Study:

  • To investigate the mechanisms of apoptosis in nerve biopsy specimens from patients with vasculitic neuropathy.
  • To determine the role of secretory and nonsecretory pathways in T-cell-mediated apoptosis in this condition.

Main Methods:

  • Immunohistochemistry was used to detect TIA-1, granzyme A, granzyme B, Fas, and Fas-ligand expression in nerve biopsies.
  • Terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) was employed to identify apoptotic cells.
  • 19 cases of vasculitic neuropathy and 8 controls were analyzed.

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Main Results:

  • Perivascular mononuclear cells in vasculitic neuropathy expressed TIA-1, granzyme A, and granzyme B.
  • Fas and Fas-ligand expressing mononuclear cells were observed in an epineurial-perivascular distribution.
  • TUNEL-positive cells, indicating apoptosis, were identified as mononuclear cells.

Conclusions:

  • Both secretory (granzyme/TIA-1) and nonsecretory (Fas/Fas-ligand) apoptotic pathways are implicated in vasculitic neuropathy.
  • Apoptosis appears restricted to inflammatory mononuclear cells, suggesting a crucial role in the recovery process of vasculitic neuropathy.