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Updated: Aug 18, 2026

A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii
Published on: August 8, 2019
[Separation and purification of taxol using normal- and reversed-phase chromatography in tandem]
Abstract:
A method is described for separation and purification of taxol from the extract of Taxus cuspidate callus culture with Al2O3 normal-phase chromatography and C18 reversed-phase chromatography, The operating conditions of the two chromatographic steps were investigated and optimized. The content of taxol was greatly increased after Al2O3 chromatography. The purity of taxol reached 95% from less than 1.0% after the two chromatographic steps. The final purity of taxol was more than 98% after the process of crystallization. The 13CNMR spectrum of the product was identical to that of taxol reported in the literature.
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