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Updated: Jul 11, 2026

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
Published on: July 28, 2010
[Construction and expression of anticolonic cancer scFv fragment]
Abstract:
Anticolonic cancer scFv fragment with a VH-linker-VL structure was constructed and expressed in E. coli. SDS-PAGE analysis showed the fragment cloned in pComb3 was not expressed efficiently in JM83, while cloned in pET-22b(+) highly expressed in BL21(DE3) up to 35.5% of the total bacterial protein obtained, if the culture was incubated under 30 degrees C.
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