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Related Experiment Videos

Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal

M Ohsawa1, S L Dun, L F Tseng

  • 1Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI 52226, USA.

European Journal of Pharmacology
|July 8, 2000
PubMed
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The cocaine- and amphetamine-regulated transcript (CART) peptide is found in the mouse spinal cord. Intrathecal administration of CART peptide causes hyperalgesia, indicated by reduced paw withdrawal latency.

Area of Science:

  • Neuroscience
  • Pain Research
  • Molecular Biology

Background:

  • The cocaine- and amphetamine-regulated transcript (CART) peptide is implicated in various physiological processes.
  • Its role in pain modulation within the central nervous system, particularly the dorsal horn, requires further elucidation.

Purpose of the Study:

  • To investigate the distribution of CART-immunoreactive fibers in the mouse dorsal horn.
  • To determine the effect of intrathecal administration of the CART peptide-(55-102) on pain sensitivity in mice.

Main Methods:

  • Immunohistochemistry was employed to visualize CART-immunoreactive structures in mouse spinal cord sections.
  • The effect of intrathecal injection of CART peptide-(55-102) or saline on paw withdrawal latency was assessed in a behavioral pain test.

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Main Results:

  • CART-immunoreactive fibers were abundant in laminae I and II of the mouse dorsal horn, with scattered positive somata.
  • Intrathecal injection of CART peptide-(55-102) resulted in a dose-dependent and significant decrease in paw withdrawal latency.
  • Saline injection did not produce significant changes in paw withdrawal latency.

Conclusions:

  • This study provides the first evidence of CART-immunoreactive fibers in the dorsal horn of the mouse spinal cord.
  • Intrathecal administration of CART peptide-(55-102) induces hyperalgesia in mice, as measured by reduced paw withdrawal latency.