Tumor-host cell hybrids in radiochimeras reconstituted with bone marrow and thymus grafts

CBA/H and CBA/HT676 radiation chimeras were prepared by lethal irradiation and subsequent reconstitution with bone marrow of the host karyotype and thymocytes of the opposite karyotype. After T- and B-cell chimerism had been established, the animals were inoculated with SEWA or TA3Ha ascites tumor--donor cell hybrids were isolated following the explantation of TA3Ha tumor in selective HAT medium and by selecting for adherent cells from SEWA. The T6T6 chromosomal marker served to distinguish between the type of cell involved in the fusion. In all hybrids the donor component was a nonthymus-derived cell.