Related Experiment Videos
Growth factor receptor signalling: location, location, location
1Depts of Medicine and Biochemistry and Molecular Biology, Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, MN 55905, USA. leof.edward@mayo.edu
Abstract:
Ligand binding to plasma membrane receptors initiates a series of events culminating in a variety of changes in cellular phenotypes. Although numerous publications have documented the activation/inactivation of signalling molecules following receptor binding, relatively few investigations have focused on the cellular compartment responsible for either initiating or selecting the particular pathway that mediates the response. Specifically, does receptor signalling occur only at the plasma membrane; is signalling dependent upon the location of defined endosome populations; or are components of both plasma membrane and endosomal activity operative depending upon the particular signalling pathway or cell type? This review addresses aspects of these questions by discussing the evidence supporting or contrasting the interplay between the endocytic and signalling systems for a subset of tyrosine kinase, serine/threonine kinase and G-protein-coupled receptors.
Insights
Receptor signaling location is explored, questioning if it occurs solely at the plasma membrane or involves endosomes. This review examines the interplay between endocytic and signaling systems for various receptor types.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Plasma membrane receptor binding triggers cellular phenotype changes.
- Limited research focuses on the specific cellular compartments initiating or selecting signaling pathways.
- Key questions involve the roles of the plasma membrane versus endosomes in receptor-mediated signaling.
Purpose of the Study:
- To investigate the cellular location of receptor-mediated signaling initiation and pathway selection.
- To explore the interplay between the endocytic system and signaling pathways.
- To review evidence concerning plasma membrane and endosomal contributions to signaling.
Main Methods:
- Literature review and synthesis of existing research.
- Discussion of evidence for various receptor classes: tyrosine kinase, serine/threonine kinase, and G-protein-coupled receptors.
- Comparative analysis of signaling mechanisms at the plasma membrane and endosomes.
Main Results:
- Evidence suggests signaling can occur at both the plasma membrane and endosomes.
- The specific location and pathway depend on the receptor type and cell context.
- The endocytic system plays a dynamic role in modulating receptor signaling.
Conclusions:
- Receptor signaling is not confined to the plasma membrane; endosomes are critical signaling platforms.
- The integration of plasma membrane and endosomal signaling determines cellular responses.
- Understanding this interplay is crucial for deciphering complex cellular signaling networks.