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Related Experiment Videos

CDw108 expression during T-cell development.

T Mine1, K Harada, T Matsumoto

  • 1Kurume University Research Center for Innovative Cancer Therapy, Department of Surgery, Kurume University School of Medicine, Japan.

Tissue Antigens
|July 8, 2000
PubMed
Summary
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CDw108, a T lymphocyte glycoprotein, is expressed on stem cells and developing T cells in the thymus. Its expression suggests a role in T-cell development, particularly during positive selection.

Area of Science:

  • Immunology
  • Developmental Biology
  • Molecular Biology

Background:

  • CDw108 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein.
  • It is primarily expressed on activated T lymphocytes and erythrocytes.

Purpose of the Study:

  • Investigate CDw108 expression in various tissues and cells.
  • Determine the role of CDw108 in T-cell development, especially during thymic positive selection.

Main Methods:

  • Cloned murine CDw108 cDNA.
  • Analyzed CDw108 mRNA expression in tissues using RT-PCR.
  • Examined cell-surface CDw108 expression on human umbilical cord blood and thymus cells via flow cytometry.

Main Results:

  • Murine CDw108 cDNA showed high homology to human CDw108.

Related Experiment Videos

  • CDw108 mRNA was detected in thymus and brain, with peak expression in early embryonic development.
  • Cell-surface CDw108 was found on CD34+ stem cells in umbilical cord blood and specific thymocyte populations during positive selection.

    • Conclusions:

    • CDw108 is expressed during T-cell development.
    • The expression pattern suggests CDw108 contributes to T-cell development, particularly during thymic positive selection.