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Cryoconservation--archiving for the future.

P H Glenister1, C E Thornton

  • 1MRC Mammalian Genetics Unit, Harwell, Oxon OX11 ORD, UK. P.Glenister@har.mrc.ac.uk

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|July 25, 2000
PubMed
Summary

Cryopreservation of mouse embryos and spermatozoa is crucial for archiving mouse stocks and disseminating new strains. This method supports efficient gene function studies and genetic mapping in mammalian genetics.

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Area of Science:

  • Mammalian genetics
  • Gene function studies
  • Mouse models

Background:

  • Systematic mutagenesis approaches are essential for studying mammalian gene function post-genome.
  • Expanding mouse mutant resources necessitate efficient archiving and dissemination strategies.
  • Current animal facility space may be insufficient for growing mouse mutant collections.

Purpose of the Study:

  • To highlight the critical role of cryopreservation in managing expanding mouse mutant resources.
  • To discuss the application of cryopreservation for archiving, dissemination, and genetic mapping.
  • To explore the potential of cryopreserving oocytes and ovaries.

Main Methods:

  • Cryopreservation of mouse embryos and spermatozoa.
  • Archiving and dissemination of mouse strains using frozen samples.
  • Production of backcross progeny for genetic mapping from frozen spermatozoa.

Main Results:

  • Cryopreservation enables efficient archiving and distribution of mouse stocks.
  • Frozen spermatozoa facilitate rapid genetic mapping through backcross progeny production.
  • Oocytes and ovaries represent potential additions to current cryopreservation methods.

Conclusions:

  • Comprehensive mouse mutant archives are vital for 21st-century mammalian genetics.
  • Cryopreservation strategies are indispensable for managing and utilizing mouse genetic resources.
  • The strategic use of cryopreserved materials enhances research efficiency and accessibility.

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