Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction between neurone and microglia mediated by platelet-activating factor.

M Aihara1, S Ishii, K Kume

  • 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Tokyo, Tokyo 113-0033, and CREST of Japan Science and Technology Incorporation, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|July 11, 2000
PubMed
Summary

Platelet-activating factor (PAF) is synthesized by neurons when stimulated by glutamate. Microglia respond to PAF through a receptor-mediated process, highlighting PAF's role in neuron-microglia communication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum thymus and activation-regulated chemokine (TARC/CCL17) may be useful to predict the disease activity in patients with bullous pemphigoid.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

Leptin induces interleukin-6 production in keratinocytes via decreased expression of caveolin-1: a possible link between obesity and psoriatic inflammation.

The British journal of dermatology·2020
Same author

A comparison of the effect of large and small metal-on-metal bearings in total hip arthroplasty on metal ion levels and the incidence of pseudotumour: a five-year follow-up of a previous report.

The bone & joint journal·2018
Same author

Genetic correlations between the cumulative pseudo-survival rate, milk yield, and somatic cell score during lactation in Holstein cattle in Japan using a random regression model.

Journal of dairy science·2017
Same author

Elevation of serum squamous cell carcinoma antigen 2 in patients with psoriasis: associations with disease severity and response to the treatment.

The British journal of dermatology·2016
Same author

Fish collagen is an important panallergen in the Japanese population.

Allergy·2016

Area of Science:

  • Neuroscience
  • Cell Biology
  • Neuroimmunology

Background:

  • Platelet-activating factor (PAF) is a significant phospholipid mediator involved in neuronal function and brain development.
  • PAF production and release occur in the brain during pathological conditions.
  • The specific cells and mechanisms of PAF synthesis and its target cells in the brain remain unclear.

Purpose of the Study:

  • To identify the cells responsible for PAF synthesis in the brain.
  • To elucidate the mechanisms underlying PAF synthesis and its signaling pathways.
  • To investigate the role of PAF in neuron-microglial interactions.

Main Methods:

  • Primary rat brain cell cultures were utilized.
  • A highly sensitive assay method was employed to detect PAF.

Related Experiment Videos

  • Experiments involved stimulation with glutamic acid and assessment of microglial chemotaxis.
  • Studies included the use of PAF-receptor-deficient mice and pertussis toxin.

    • Main Results:

    • Neurons synthesize PAF upon stimulation with glutamic acid, dependent on NMDA receptor activation and calcium influx.
    • Microglia exhibit a strong chemotactic response to PAF, mediated by functional PAF receptors.
    • This microglial response involves a pertussis-toxin-sensitive G-protein and mitogen-activated protein kinase signaling.

    Conclusions:

    • PAF acts as a crucial signaling molecule in the communication between neurons and microglia.
    • Understanding PAF's role is important given the dual cytoprotective and cytotoxic functions of microglia.