Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional adenosine receptors in human corpora cavernosa.

S Filippi1, M Mancini, S Amerini

  • 1Department of Pharmacology, University of Florence, Florence, Italy.

International Journal of Andrology
|July 25, 2000
PubMed
Summary

Adenosine relaxes human penile vessels, including the corpus cavernosum and veins, but does not cause erection. Prostaglandin E1 (PGE1) causes erection by relaxing the corpus cavernosum and contracting veins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Architecture and regulation of a GDNF-GFRα1 synaptic adhesion assembly.

Nature communications·2023
Same author

Acyl-CoA binding protein (ACBP) is highly expressed in the developing human kidney.

European review for medical and pharmacological sciences·2022
Same author

Fetal programming of COVID-19: may the barker hypothesis explain the susceptibility of a subset of young adults to develop severe disease?

European review for medical and pharmacological sciences·2021
Same author

The novel collagen matrikine, endotrophin, is associated with mortality and cardiovascular events in patients with atherosclerosis.

Journal of internal medicine·2021
Same author

Molecular pathways triggered by COVID-19 in different organs: ACE2 receptor-expressing cells under attack? A review.

European review for medical and pharmacological sciences·2020
Same author

Corrigendum. Sequencing of a 'mouse azoospermia' gene panel in azoospermic men: identification of RNF212 and STAG3 mutations as novel genetic causes of meiotic arrest.

Human reproduction (Oxford, England)·2020

Area of Science:

  • Pharmacology
  • Urology
  • Physiology

Background:

  • Adenosine exhibits relaxant activity on rabbit corpus cavernosum via A2a receptors.
  • Understanding adenosine receptor function in human penile vasculature is crucial for erectile dysfunction research.

Purpose of the Study:

  • To identify and functionally characterize adenosine receptors in human penile vessels.
  • To investigate the in vivo and in vitro effects of adenosine on penile hemodynamics and erectile response.
  • To compare the effects of adenosine with prostaglandin E1 (PGE1) on penile tissues.

Main Methods:

  • Binding studies using a selective radioligand ([125I]PAPA-APEC) to identify A2 receptors in human corpora cavernosa (HCC).
  • Functional studies assessing adenosine-induced relaxation in phenylephrine-precontracted HCC.

Related Experiment Videos

  • In vivo administration of adenosine and PGE1 into the corpora cavernosa of healthy volunteers, monitoring blood flow and erectile response.
  • In vitro experiments on human deep dorsal penile veins (DDPV) to assess adenosine and PGE1 effects on vascular tone.

    • Main Results:

    • High-affinity, low-capacity adenosine A2 receptors were identified in HCC membranes.
    • Adenosine demonstrated potent, nitric oxide-independent relaxation of HCC, counteracted by an A2 antagonist.
    • In vivo, adenosine increased cavernosal blood flow dose-dependently but did not induce erection.
    • Adenosine relaxed noradrenaline-precontracted human deep dorsal penile veins, while PGE1 caused vein contraction.

    Conclusions:

    • Adenosine relaxes both human corpus cavernosum and penile veins.
    • The lack of erection with adenosine may be due to simultaneous relaxation of both corpora cavernosa and veins.
    • PGE1 induces erection by relaxing the corpus cavernosum and contracting penile veins, contrasting with adenosine's effects.