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Updated: Oct 15, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
10:17

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

Ten microsatellite loci isolated and developed for the blackspot seabream, Pagellus bogaraveo (Brünnich 1768)

B M Stockley1, A D Rogers, A Iyengar

  • 1School of Ocean and Earth Sciences, Southampton University, Southampton Oceanography Centre, European Way, Southampton SO14 3ZH, UK. bruce@soton.ac.uk

Molecular Ecology
|July 8, 2000
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...