Related Experiment Video
Updated: Aug 10, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7
O V Bystrova1, G V Zatonskii, S A Borisova
1Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. knirel@ioc.ac.ru
Abstract:
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: [figure], where Rhap2Ac is 2-O-acetylrhamnopyranose.
More Related Videos
09:06Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Published on: February 22, 2020
10:24Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Published on: April 10, 2020
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Peptidoglycan Synthesis
Formation of Lipopolysaccharides
Bacterial Phylum Actinobacteria
Archaeal Cell Wall