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[Determination of the minimum inhibitory concentration of fluconazole against Cryptococcus neoformans]

C M Fernández Andreu1, T Pimentel Turino, G Martínez Machín

  • 1ciipk@infomed.sld.cu

Revista Cubana De Medicina Tropical
|July 11, 2000
PubMed

Insights

This study determined fluconazole

Area of Science:

  • Medical Mycology
  • Antimicrobial Resistance

Background:

  • Cryptococcus neoformans meningitis is a severe opportunistic infection, particularly in AIDS patients.
  • Fluconazole is a primary antifungal treatment in many regions.
  • Monitoring antifungal susceptibility is crucial for effective treatment.

Purpose of the Study:

  • To determine the minimum inhibitory concentration (MIC) of fluconazole against Cryptococcus neoformans var. neoformans.
  • To assess the in vitro susceptibility of clinical isolates to a widely used antifungal agent.

Main Methods:

  • Casitone broth microdilution method was employed.
  • 36 strains of Cryptococcus neoformans var. neoformans were tested.
  • Minimum Inhibitory Concentrations (MICs) were quantified.

Main Results:

  • MICs ranged from 0.125 to >64 µg/mL, with a geometric mean of 2.38 µg/mL.
  • 50% of strains were inhibited by 4 µg/mL.
  • The minimum concentration inhibiting 90% of strains (MIC90) was 16 µg/mL.

Conclusions:

  • In vitro fluconazole susceptibility varies among Cryptococcus neoformans strains.
  • Continued surveillance for antifungal resistance in C. neoformans is essential.
  • These findings inform treatment strategies for cryptococcal meningitis.

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