Related Experiment Video
Updated: Aug 11, 2026
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
Published on: July 1, 2011
[Serologic diagnosis of human leptospirosis with 3 variants of the passive hemagglutination technique]
A M Obregón Fuentes1, M Martell
1ciipk@infomed.sld.cu
A serological study was conducted to evaluate 3 variants of the positive hemagglutination technique (HA) with the use of formalized erythrocytes of fresh lamb (HC) and of formalized Type O, Rh-negative human red blood cells (HH). No significant statistical difference were found between the results of HA and HC and between HC and HH. Unspecific reaction was only observed in 2 of the 200 sera studied by HH and HC. Heterophil antibodies were just observed in 2.5% of the sera studied by HH, and in 100% of the sera studied by HC. The qualitative parameters were determined for the variants used, and this way the repetition and reproducibility of the most useful variant was evaluated.
A serological study was conducted to evaluate 3 variants of the positive hemagglutination technique (HA) with the use of formalized erythrocytes of fresh lamb (HC) and of formalized Type O, Rh-negative human red blood cells (HH). No significant statistical difference were found between the results of HA and HC and between HC and HH. Unspecific reaction was only observed in 2 of the 200 sera studied by HH and HC. Heterophil antibodies were just observed in 2.5% of the sera studied by HH, and in 100% of the sera studied by HC. The qualitative parameters were determined for the variants used, and this way the repetition and reproducibility of the most useful variant was evaluated.
