[The conservative region sequence analysis of PdSox9 in Paramisgurnus dabryanus]

Degenerate primers within HMG-box analyzed the Sox genes of P. dabryanus. Three amplification bands with the length 220, 550 and 1500 bp respectively were observed. A novel gene was obtained from the 550 bp band. The identity of possible amino acid sequence to Sox9 of human, mouse and chicken is 94%. The similarity to HSOX10, MSox10, HSRY, MSry is 87%, 55%, 40% and 47% respectively. So, it was named PdSox9. Northern blotting result showed that it overexposes in adult testis and brain.