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[Construction of silkworm RAPD molecular linkage map]
1Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing, China.
Yi Chuan Xue Bao = Acta Genetica Sinica
|July 11, 2000
Summary
Researchers constructed a Random Amplified Polymorphic DNA (RAPD) linkage map for Bombyx mori, detailing 182 loci across 39 linkage groups spanning over 1,148 cM genetic distance.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Entomology
Context:
- Bombyx mori (silkworm) is an economically important insect model.
- Accurate genetic maps are crucial for understanding gene organization and facilitating breeding programs.
- Previous mapping efforts provide a foundation for integrated map construction.
Purpose:
- To construct a high-density Random Amplified Polymorphic DNA (RAPD) linkage map for Bombyx mori.
- To identify and map 182 RAPD loci across 39 linkage groups.
- To establish a genetic map covering a total genetic distance exceeding 1,148.3 centimorgans (cM).
Summary:
- A novel RAPD linkage map of Bombyx mori was developed using the Dazao/C108 cross and their F2 generation.
- The map comprises 182 RAPD loci, with 103 derived from Dazao (23 linkage groups) and 79 from C108 (16 linkage groups).
- The total genetic distance covered by this map is over 1,148.3 cM, providing a comprehensive genetic framework.
Impact:
- This map serves as a valuable resource for future genetic and genomic studies in Bombyx mori.
- It can be integrated with existing maps, such as the SADF and RFLP maps, for a more robust genetic analysis.
- Facilitates gene mapping, marker-assisted selection, and understanding of silkworm genetics and evolution.