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Updated: Oct 15, 2026

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Inhibition of microtubule polymerization by SK&F 96365, a blocker of receptor-linked Ca2+ entry
M Mitsui-Saito1, N Nakahata, Y Ohizumi
1Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Abstract:
SK&F 96365 (1-[beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl]-1H-imid azole hydrochloride) is widely used as an effective inhibitor of receptor-linked and capacitative Ca2+ entry. Since this inhibitor has additional effects such as inhibition of voltage-dependent Ca2+ channels, sarco- and endoplasmic reticula Ca2+ pumps and cell proliferation, its molecular mechanism of action remains to be solved. In the present study, we have investigated the effect of SK&F 96365 on microtubule protein isolated from bovine brain in vitro. SK&F 96365 depolymerized the polymerized microtubules in a concentration-dependent manner. This result suggests that SK&F 96365 directly depolymerizes microtubules, an effect that may contribute to the various actions of this compound.
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