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Correction for quenching in fluorimetric determinations using steady state fluorescence.

S Maji¹, K Sundararajan, K S Viswanathan

¹Materials Chemistry Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, India.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|July 11, 2000

Summary

This study introduces a new method for correcting fluorescence quenching in analytical chemistry. It uses steady-state fluorescence spectroscopy to accurately measure analytes even with interfering substances present.

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Area of Science:

Analytical Chemistry
Spectroscopy

Background:

Fluorescence quenching can interfere with quantitative analysis.
Time-resolved fluorometry is a common method for correction.
A simpler method using steady-state fluorescence is needed.

Purpose of the Study:

To develop a novel procedure for quenching correction in fluorimetric determinations.
To utilize steady-state fluorescence spectroscopy for this correction.
To demonstrate the method's applicability in determining uranium.

Main Methods:

Employing steady-state fluorescence spectroscopy.
Analyzing the variation in the slope of fluorescence versus concentration plots.
Investigating the effect of changing quencher concentrations.

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Main Results:

A new procedure for quenching correction was successfully developed.
The method effectively corrects for quenching effects.
The determination of uranium in the presence of metal ion quenchers was demonstrated.

Conclusions:

Steady-state fluorescence spectroscopy offers a viable alternative for quenching correction.
This method provides accurate results in the presence of quenchers.
The developed procedure is applicable to various analytical determinations.