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Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you
W Lukowitz1, C S Gillmor, W R Scheible
1Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305, USA.
Plant Physiology
|July 13, 2000
Abstract:
Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.