Detection of protein binding to a glucocorticoid response element-like sequence in a chicken major histocompatibility...

The glucocorticoid response element in gene promoters mediates regulation of gene expression by glucocorticoids. The major histocompatibility (MHC) class II genes, crucial for immunoresponsiveness, are among those modulated by glucocorticoids. A GRE-like sequence has been located in the promoter of a chicken MHC class II promoter. DNase footprinting revealed protein binding by the GRE-like sequence when nuclear extract from chicken T or B cell lines were used. Gel shift assays detected multiple binding activities in the lymphocyte cell lines, but little binding in the macrophage cell line. Relative band intensity differed among the lymphocyte cell lines. By using a mutant GRE oligonucleotide, most of the binding activities were demonstrated to be specific to the GRE. This study suggests a role of the GRE-like sequence in regulating chicken MHC class II genes and provides further evidence for the previously reported influence of glucocorticoids on chicken MHC class II expression which may be the molecular basis of glucocorticoid immunomodulation.