Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peroxisome proliferators induce apoptosis and decrease DNA synthesis in hepatoma cell lines.

V Goll1, C Viollon-Abadie, L Nicod

  • 1Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France.

Human & Experimental Toxicology
|July 13, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcome of treatment of MDR-TB or drug-resistant patients treated with bedaquiline and delamanid: Results from a large global cohort.

Pulmonology·2021
Same author

A Randomized Placebo-Controlled Efficacy Study of a Prime Boost Therapeutic Vaccination Strategy in HIV-1-Infected Individuals: VRI02 ANRS 149 LIGHT Phase II Trial.

Journal of virology·2021
Same author

[Handling of allergen immunotherapy in the COVID-19 pandemic: An ARIA-EAACI-AeDA-GPA-DGAKI Position Paper (Pocket-Guide)].

Laryngo- rhino- otologie·2020
Same author

Whole-genome sequencing for rapid, reliable and routine investigation of <i>Mycobacterium tuberculosis</i> transmission in local communities.

New microbes and new infections·2019
Same author

[COLT: Ten years of research in lung transplantation, results and perspectives].

Revue des maladies respiratoires·2018
Same author

Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL-based 3D scaffold.

International endodontic journal·2017
Same journal

Establishment of a rat model of bronchopulmonary dysplasia complicated with pulmonary hypertension and its potential applications.

Human & experimental toxicology·2026
Same journal

Pulmonary iron oxide (Fe<sub>3</sub>O<sub>4</sub>) nanoparticle-biomolecule interactions modified during aging and metabolic syndrome disease progression.

Human & experimental toxicology·2026
Same journal

Retraction: Carvedilol alleviates the detrimental effects of azathioprine on hepatic tissues in experimental rats: Focusing on redox system, inflammatory and apoptosis pathways.

Human & experimental toxicology·2026
Same journal

Inhibition of store-operated calcium entry mitigates lipopolysaccharide-induced hepatotoxicity via modulating inflammatory and oxidative stress responses.

Human & experimental toxicology·2026
Same journal

Stevioside inhibits colorectal cancer progression by regulating macrophage polarization.

Human & experimental toxicology·2026
Same journal

Predictive factors of severe outcome in glufosinate ammonium poisoning: A retrospective study in Vietnam.

Human & experimental toxicology·2025
See all related articles

Peroxisome proliferators (PPs) increased enzyme activity and apoptosis in rat liver cells but had varied effects on human liver cells. These findings contrast with previous studies on primary hepatocytes.

Area of Science:

  • Hepatology
  • Toxicology
  • Cell Biology

Background:

  • Peroxisome proliferators (PPs) are compounds known to affect liver cell function.
  • Previous studies indicated PPs alter enzyme activity, apoptosis, and DNA synthesis in primary hepatocytes.

Purpose of the Study:

  • To investigate the impact of various PPs on peroxisomal enzyme activity, apoptosis, and DNA synthesis in rat FaO and human HepG2 hepatoma cell lines.
  • To compare the cellular responses in hepatoma cell lines with those observed in primary hepatocyte cultures.

Main Methods:

  • Treatment of rat FaO and human HepG2 hepatoma cell lines with PPs (clofibric acid, bezafibrate, ciprofibrate, nafenopin, DEHP) at 250 microM for 48 or 72 hours.
  • Assay of peroxisomal enzyme activities, apoptosis markers, and DNA synthesis.

Related Experiment Videos

  • Evaluation of effects on both growing and confluent cell cultures.

    • Main Results:

    • PPs increased peroxisomal enzyme activities in rat FaO cells but not in human HepG2 cells.
    • PPs strongly induced apoptosis in FaO cells, with some exceptions for DEHP and NAFE regarding TGFbeta-induced apoptosis in confluent cultures.
    • PPs decreased DNA synthesis in FaO cells and growing HepG2 cells, while CLO, CIPRO, and NAFE induced apoptosis in confluent HepG2 cultures.

    Conclusions:

    • Hepatoma cell lines exhibit differential responses to PPs compared to primary hepatocytes.
    • PPs demonstrate cell-type-specific effects on peroxisomal activity, apoptosis, and DNA synthesis.
    • The study highlights the importance of cell context in understanding PP-induced toxicity.