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Related Experiment Videos

pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.

R P Hellens1, E A Edwards, N R Leyland

  • 1John Innes Centre, Norwich Research Park, Colney, UK. hellens@bbsrc.ac.uk

Plant Molecular Biology
|July 13, 2000
PubMed
Summary

The pGreen plasmid system offers versatile binary Ti vectors for Agrobacterium-mediated plant transformation. This system enhances cloning flexibility and transformation efficiency across diverse plant species.

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Plant molecular biology·2013

Area of Science:

  • Molecular Biology
  • Plant Biotechnology

Background:

  • Binary Ti vectors are crucial for Agrobacterium-mediated plant transformation.
  • The pGreen series provides user-friendly binary Ti vectors for various plant transformation protocols.

Purpose of the Study:

  • To introduce the pGreen plasmid system for enhanced plant transformation.
  • To highlight the flexibility and efficiency of pGreen vectors.

Main Methods:

  • Utilizing pGreen binary Ti vectors in Agrobacterium-mediated transformation.
  • Leveraging pSoup for pGreen replication in Agrobacterium.
  • Employing pBluescript-based cloning sites for genetic manipulation.

Main Results:

  • pGreen vectors allow flexible gene arrangement at T-DNA borders.

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  • The system demonstrates efficient transformation in multiple plant species.
  • Optimized size and copy number in E. coli facilitate in vitro recombination.

    • Conclusions:

    • The pGreen system offers a flexible and efficient tool for plant genetic engineering.
    • pGreen vectors are comparable in efficiency to existing binary Ti vectors.
    • An online resource provides comprehensive information and protocols for the pGreen system.