Related Experiment Videos
Rapid screening of natural products by high-resolution high-temperature gas chromatography
S A Carbonell1, F R de Aquino Neto, J N Cardoso
1LADETEC, Instituto de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitaria, Rio de Janeiro, Brazil.
Abstract:
The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.