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Sensitive method for the determination of ambroxol in body fluids by capillary electrophoresis and fluorescence
T Perez-Ruiz1, C Martínez-Lozano, A Sanz
1Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain. tpr@fcu.um.es
Abstract:
A sensitive and rapid capillary electrophoretic method combined with laser-induced fluorescence detection has been developed for the determination of ambroxol. Samples were derivatized with 5 x 10(-4) M fluorescein isothiocyanate. A linear relationship between concentration and peak area was obtained in the concentration range 0.008-42 microg ml(-1) with a correlation coefficient of 0.9999. The applicability of the method to serum and urine samples was demonstrated. The method is also useful for the determination of ambroxol in pharmaceutical preparations.