Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automatic poisson peak harvesting for high throughput protein identification.

E J Breen1, F G Hopwood, K L Williams

  • 1Proteome Systems Ltd., North Ryde, NSW, Australia. ed.breen@proteomesystems.com

Electrophoresis
|July 13, 2000
PubMed
Summary

This study introduces an automated peak harvester for mass spectrometry, improving high-throughput protein identification. The tool accurately identifies monoisotopic peptide masses, matching human operator performance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient-specific modelling of pulmonary arterial hypertension: wall shear stress correlates with disease severity.

Frontiers in bioengineering and biotechnology·2025
Same author

Optimizing genomic prediction for Australian Red dairy cattle.

Journal of dairy science·2020
Same author

Non-canonical cyclic AMP SMAD1/5/8 signalling in human granulosa cells.

Molecular and cellular endocrinology·2019
Same author

Pulmonary arterial hypertension - progress in understanding the disease and prioritizing strategies for drug development.

Journal of internal medicine·2017
Same author

The genotype-phenotype landscape of familial amyotrophic lateral sclerosis in Australia.

Clinical genetics·2017
Same author

Phenotypic differentiation is associated with divergent sexual selection among closely related barn swallow populations.

Journal of evolutionary biology·2016

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • High-throughput protein identification relies on accurate peptide mass fingerprinting.
  • Automated peak picking from mass spectra is crucial for efficiency.

Purpose of the Study:

  • To develop an automated peak harvester for identifying monoisotopic peptide masses from MALDI-TOF mass spectra.
  • To enhance the efficiency and accuracy of protein identification workflows.

Main Methods:

  • Utilized mathematical morphology and watershed algorithms for spectral processing.
  • Applied Poisson modeling to determine monoisotopic peaks within isotopic distributions.
  • Tested on standard peptides, bovine serum albumin, and E. coli proteins.

Related Experiment Videos

Main Results:

  • The peak harvester effectively identified monoisotopic peaks, comparable to experienced human operators.
  • Successfully resolved overlapping isotopic distributions for accurate mass identification.
  • Demonstrated effectiveness across diverse protein samples and experimental conditions.

Conclusions:

  • The developed peak harvester automates monoisotopic peak picking in MALDI-TOF mass spectrometry.
  • This tool significantly contributes to high-throughput automated protein identification.
  • It offers interactive and fully automated modes for integration into existing workflows.