The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates
R Kierzek1, M A Steiger, S L Spinelli
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Abstract:
A phosphoramidite, solid support method for the chemical synthesis of oligoribonucleotides containing 2'-O-phosphate at a selected position is presented. Synthesis of these oligoribonucleotides is based on uridine- and adenosine-(2'-O-phosphate)-3'-phosphoramidites, and a new condition for removal of 2'-O-phosphate protecting groups, which does not cleave internucleotide bonds. The structure of oligoribonucleotides with 2'-O-phosphate has been proven by enzymatic digestions and dephosphorylation by yeast 2'-phosphotransferase.
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