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The nonenzymatic hydrolysis of oligoribonucleotides. VII. Structural elements affecting hydrolysis
A Bibillo1, M Figlerowicz, K Ziomek
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Nucleosides, Nucleotides & Nucleic Acids
|July 14, 2000
Abstract:
Several elements of oligoribonucleotide structure are important for efficient hydrolysis. We have found that the following factors influence oligoribonucleotide hydrolysis: (i) single-stranded structure of RNA flanking the scissile phosphodiester bond, (ii) the substituent on atom C-5 of the uridine adjacent to the cleaved internucleotide bond, (iii) the position of the scissile UA phosphodiester bond within a hairpin loop, (iv) the concentration of formamide, urea, ethanol and sodium chloride.