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Human glutathione-S-transferase: cloning and expression in Lactococcus lactis
Biomolecular Engineering
|July 14, 2000
Abstract:
The glutathione-S-transferase A1 cDNA was amplified from the total RNAs of human liver by RT-PCR, and inserted into plasmid pMG36e. The hGSTA1 was expressed in Lactococcus lactis MG1363 and verified by SDS-PAGE and Western blot, purified by affinity chromatography and showed enzymatic activity.

