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Extracellular bicarbonate ions and insulin secretion.

J C Henquin, A E Lambert

    Biochimica Et Biophysica Acta
    |February 13, 1975
    PubMed
    Summary

    Bicarbonate ions are crucial for glucose-stimulated insulin release from rat islets. Its absence abolishes early insulin secretion and significantly inhibits the second phase, highlighting bicarbonate

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    Area of Science:

    • Endocrinology
    • Metabolic Research

    Background:

    • Insulin secretion is a complex process regulated by various ions.
    • The specific role of bicarbonate ions in insulin release requires further elucidation.

    Purpose of the Study:

    • To investigate the essential role of bicarbonate ions (HCO3-) in glucose-induced insulin secretion.
    • To determine the impact of bicarbonate absence on different phases of insulin release and responses to other secretagogues.

    Main Methods:

    • Utilized incubated and perifused isolated rat islets of Langerhans.
    • Manipulated the presence and absence of sodium bicarbonate (NaHCO3) in the incubation medium.
    • Assessed insulin release in response to glucose, tolbutamide, and high potassium (K+).

    Main Results:

    • Absence of NaHCO3 completely abolished the early phase of glucose-induced insulin secretion.
    • The second phase of glucose-induced insulin secretion was inhibited by approximately 65% without NaHCO3.
    • Insulin secretion in response to tolbutamide was markedly diminished, while K+-evoked release was minimally affected.
    • Low concentrations of bicarbonate (3-5mM) were sufficient to restore normal glucose response.

    Conclusions:

    • Bicarbonate ions are indispensable for both early and late phases of glucose-stimulated insulin secretion.
    • Bicarbonate plays a specific role in mediating insulinotropic effects of glucose and tolbutamide, but not high K+.
    • The findings underscore the critical importance of bicarbonate in pancreatic beta-cell function and insulin homeostasis.

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