Related Experiment Video
Updated: Oct 15, 2026
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
Measurement of rabbit apolipoprotein B by use of electroimmunodiffusion and immunonephelometric assays
A Tailleux1, J Labbe, C Mallet
1Département d'Athérosclérose et INSERM U. 325, Institut Pasteur de Lille, France.
Background And Purpose:
Because of their similarity to humans, rabbits are a good animal model for the study of atherosclerosis associated with high serum low-density lipoprotein (LDL) values. Two assays were developed to measure apolipoprotein B (apoB), the major structural and functional apolipoprotein of LDL in rabbits and to distinguish endogenous LDL in transgenic rabbits from that of human apoB.
Methods:
Two procedures, an electroimmunoassay (EIA) and an immunonephelometric assay (INA), along with a goat-origin rabbit antiserum were developed to measure serum apoB concentration in rabbits.
Results:
Use of either assay resulted in ability to measure rabbit species-specific apoB concentration.
Conclusion:
These assays should have broad applications: to screen compounds or diets that might lower serum apoB concentrations; to specifically measure human apoB concentration in transgenic rabbits; to measure serum apoB concentration in rabbits overexpressing other human proteins.
