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Cloning, structural organization, and chromosomal assignment of the porcine c-fos proto-oncogene, FOS
G Reiner1, L Heinricy, B Brenig
1Department of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, Germany. g-reiner@uni-hohenheim.de
Cytogenetics and Cell Genetics
|July 15, 2000
Abstract:
The complete porcine c-fos proto-oncogene (FOS) with flanking regions was cloned and sequenced. FOS consists of four exons at amino acids 1-47, 48-131, 132-167, and 168-380 and includes all the typical motifs of the fos proto-oncogene. The promoter contains consensus sequences for CRE, SRE, CaRE, and the E-Box, as well as an AP-1 site. Homologies between human and swine were between 89.7% and 96.3% in the exons. Based on somatic cell hybrid panel screening and known homologies between swine chromosome 7 and human chromosome 14, the porcine c-fos gene was assigned to chromosome 7q23.